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TUBA1A Monoclonal Antibody

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TUBA1A Monoclonal Antibody

貨號(hào)：

HYK-200062
規(guī)格：
圖片：
Western Blot
Positive WB detected in: U87 whole cell lysate, PC-3 whole cell lysate, 293 whole cell lysate, U251 whole cell lysate, A549 whole cell lysate, A375 whole cell lysate, MG-63 whole cell lysate, SH-SY5Y whole cell lysate,
All lanes: TUBA1A antibody at 1:5000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa

Western Blot
Positive WB detected in: Rabbit heatrt tissue, Rabbit liver tissue, Rabbit spleen tissue, Rabbit lung tissue, Rabbit kidney tissue, Rabbit small intestine tissue, Rabbit skeletal muscle tissue
All lanes: TUBA1A antibody at 1:5000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa

Western Blot
Positive WB detected in: Rat brain tissue, Rat stomach tissue, Rat kidney tissue
All lanes: TUBA1A antibody at 1:5000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa

Western Blot
Positive WB detected in: NIH/3T3 whole cell lysate, RAW264.7 whole cell lysate, Mouse brain tissue, Mouse kidney tissue
All lanes: TUBA1A antibody at 1:5000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa

Western Blot
Positive WB detected in: Hela whole cell lysate at 20μg, 10μg, 5μg, 2.5μg, 1.25μg
All lanes: TUBA1A antibody at 1:5000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa

Western Blot
Positive WB detected in: Hela whole cell lysate
All lanes: TUBA1A antibody at 1:2500, 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa

Western Blot
Positive WB detected in: Hela whole cell lysate at 20μg, 10μg, 5μg, 2.5μg, 1.25μg, 0.625μg, 0.3125μg, 0.15625μg
All lanes: Company A, Company B, Company C, Company D, CSB-MA754656A0m antibody at 1:5000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa

Western Blot
Positive WB detected in: Hela whole cell lysate
All lanes: Company A, Company B, Company C, Company D, CSB-MA754656A0m antibody at 1:2500, 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 320000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 52 kDa
Observed band size: 52 kDa

IHC image of CSB-MA754656A0m diluted at 1:150 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

IHC image of CSB-MA754656A0m diluted at 1:150 and staining in paraffin-embedded human breast cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4°C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.

Immunofluorescence staining of Hela cells with CSB-MA754656A0m at 1:75, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4°C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).

Immunoprecipitating TUBA1A in Hela whole cell lysate
Lane 1: Mouse control IgG (1μg) instead of CSB-MA754656A0m in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-MA754656A0m (5μg) + Hela whole cell lysate (500μg)
Lane 3: Hela whole cell lysate (20μg)

Immunoprecipitating TUBA1A in Hela whole cell lysate
Lane 1: Mouse control IgG (1μg) instead of CSB-MA754656A0m in Hela whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: Company A (5μg), Company B (5μg), Company C (5μg), Company D (5μg), CSB-MA754656A0m (5μg) + Hela whole cell lysate (500μg)
Lane 3: Hela whole cell lysate (20μg)

產(chǎn)品詳情

產(chǎn)品名稱：

Mouse anti-Homo sapiens (Human) TUBA1A Monoclonal Antibody antibody
Uniprot No.：

Q71U36
基因名：

TUBA1A
別名：

Alpha tubulin 3 antibody; Alpha-tubulin 3 antibody; B alpha 1 antibody; FLJ25113 antibody; LIS3 antibody; TBA1A_HUMAN antibody; TUBA1A antibody; TUBA3 antibody; Tubulin alpha 1a antibody; Tubulin alpha 1A chain antibody; Tubulin alpha 3 antibody; Tubulin alpha 3 chain antibody; Tubulin alpha brain specific antibody; Tubulin alpha-1A chain antibody; Tubulin alpha-3 chain antibody; Tubulin B alpha 1 antibody; Tubulin B-alpha-1 antibody
宿主：

Mouse
反應(yīng)種屬：

Human, Rabbit, Rat, Mouse
免疫原：

A synthesized peptide derived from human Tubulin alpha-1A chain (297-309aa)
免疫原種屬：

Homo sapiens (Human)
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal Antibody
抗體亞型：

IgG2b
純化方式：

>95%, Protein A purified
克隆號(hào)：

7E5C12
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IHC, IF, FC, IP

推薦稀釋比：

Application	Recommended Dilution
WB	1:20000-1:320000
IHC	1:100-1:300
IF	1:50-1:200
FC	1:100-1:300
IP	1μg-5μg

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunohistochemistry (IHC) Protocol
Immunofluorescence (IF) Protocol
Flow cytometry (FC) Protocol
Immunoprecipitation (IP) Protocol
儲(chǔ)存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.