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E-Tag Monoclonal Antibody

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產(chǎn)品詳情相關(guān)產(chǎn)品

E-Tag Monoclonal Antibody

貨號：

HYK-200063
規(guī)格：
圖片：
Western Blot
Positive WB detected in: E-tagged fusion protein at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng
All lanes: E-Tag antibody at 1:1000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa

Western Blot
Positive WB detected in: E-tagged fusion protein
All lanes: E-Tag antibody at 1:5000, 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000
Secondary
Goat polyclonal to Mouse IgG at 1/10000 dilution
Predicted band size: 36 kDa
Observed band size: 36 kDa

Immunoprecipitating E-Tag in 293F transfected whole cell lysate
Lane 1: Mouse control IgG (1μg) instead of CSB-MA000151M0m in 293F transfected whole cell lysate. For western blotting, a HRP-conjugated Protein G antibody was used as the secondary antibody (1/2000)
Lane 2: CSB-MA000151M0m (5μg) + 293F transfected whole cell lysate (500μg)
Lane 3: 293F transfected whole cell lysate (20μg)

ELISA E-Tag antibody in E-tagged fusion protein and Control protein (no E-tag)
Antigen at dilution of 1μg/ml
E-Tag antibody at (0-0.15μg)
Goat polyclonal to Mouse IgG at 1/50000 dilution

產(chǎn)品詳情

別名：

E-Tag
宿主：

Mouse
反應(yīng)種屬：

All
免疫原：

GAPVPYPDPLEPRC synthetic peptide conjugate to KLH
標(biāo)記方式：

Non-conjugated
克隆類型：

Monoclonal Antibody
抗體亞型：

IgG1
純化方式：

>95%, Protein G purified
克隆號：

11H12B3
濃度：

It differs from different batches. Please contact us to confirm it.
保存緩沖液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, PH 7.4
產(chǎn)品提供形式：

Liquid
應(yīng)用范圍：

ELISA, WB, IP

推薦稀釋比：

Application	Recommended Dilution
ELISA	1:4000-1:8000
WB	1:1000-1:320000
IP	1μg-5μg

Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
Immunoprecipitation (IP) Protocol
儲存條件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
貨期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.